416 Milk Components as a Tool in Predicting Tolerance in Cow's Milk Allergy
نویسندگان
چکیده
investigated by immunoblot with serum from individuals sensitized to anisakis or shrimp. IgE binding patterns in the immunoblots were used for the identification of major Anisakis allergens, which were analysed by mass spectrometry-based proteomics in ESI-Orbitrap, after separation on SDS-gel. Results: Four new allergen candidates were identified. The first identified allergen was enolase, which is related to the cockroach allergen enolase. The other allergens were Heat Shock Protein-70 (HSP 70), tubulin, and glutathion-S-transferase, which are also present as allergens in house dust mite. Conclusions: Here we describe the identification of 4 novel IgE binding allergens in anisakis. The allergens might explain IgE cross-reactivity between anisakis and house dust mite or cockroach.
منابع مشابه
Role of Cellular Immunity in Cow's Milk Allergy: Pathogenesis, Tolerance Induction, and Beyond
Food allergy is an aberrant immune-mediated reaction against harmless food substances, such as cow's milk proteins. Due to its very early introduction, cow's milk allergy is one of the earliest and most common food allergies. For this reason cow's milk allergy can be recognized as one of the first indications of an aberrant inflammatory response in early life. Classically, cow's milk allergy, a...
متن کاملAtopy patch tests are useful to predict oral tolerance in children with gastrointestinal symptoms related to non-IgE-mediated cow's milk allergy
Atopy patch tests (APTs) have been proposed for the diagnostic approach in children with non-IgE-mediated cow's milk allergy and gastrointestinal symptoms. We aimed to investigate the benefit of APTs in predicting oral tolerance in these patients. We prospectively evaluated 172 subjects with a sure diagnosis of non-IgE-mediated CMA and gastrointestinal symptoms (97 boys, 56.4%; age, 6.37 m; ran...
متن کاملDebates in allergy medicine: baked egg and milk do not accelerate tolerance to egg and milk
There is emerging evidence that children with egg and cow's milk allergy who can tolerate these allergens cooked in baked goods are more likely to develop tolerance. As a result a hypothesis has arisen that exposure to egg and milk in baked goods may hasten tolerance development; however, it is unclear whether children who develop tolerance do so because they have ingested low levels of egg or ...
متن کاملSevere anaphylaxis to sheep's milk cheese in a child desensitized to cow's milk through specific oral tolerance induction.
Specific oral tolerance induction to food (SOTI) is a new promising treatmentfor persistent IgE-mediatedfood allergy. Our paper reports a case of a 5-year-old girl with cow's milk allergy, who developed severe anaphylaxis after the ingestion of a croissant containing sheep's milk ricotta cheese, even though she had been previously desensitized to cow's milk through SOTI. The sheep's milk specif...
متن کاملAdverse reactions to milk in infants
AIM To study the age when symptoms of adverse reactions to milk occur, in premature and term children, the debut of various symptoms, immunoglobulin E (IgE)- and non-IgE-mediated reactions and the frequency of tolerance at 1 year. METHODS Six hundred and eight children, 193 premature and 416 term infants, were followed. Symptomatic children were invited to a clinical examination. The criteria...
متن کامل